Mutant analysis of Arabidopsis

Aims

The newest findings of plant hormone signal transduction and molecular mechanisms of basic developmental processes will be approached from the perspective of developmental genetics, molecular and cell biology. Course aims mainly to gain insight into the practical use of current experimental approaches and methods for study of molecular mechanisms of plant development. The main focus is on the use of a model organism Arabidopsis thaliana in investigation of hormonal regulation of plant development.

Prerequisites

Knowledge of molecular biology, genetics, plant physiology and cell biology is required.

Learning Outcomes

  Participants will ·         gain insight into hormonal network regulating plant development on cellular, tissue and organ level and future perspectives in this research field ·        gain an overview about the main methods in plant experimental biology including their advantages and limitations ·        be able to choose the most suitable experimental strategy for their scientific projects.    

Syllabus

A. Choosing the right approach - detail overview of current approaches and methods of experimental plant genetics   B. From the signal to the gene - Genetic dissection of ethylene, cytokinin and auxin signalling, molecular characterization and arrangement of the pathways   C. From the process to the mechanism – molecular mechanisms behind regulation of plant developmental processes e.g. embryogenesis, lateral root organogenesis, root meristem formation   D. Into the cell - the molecular responses on the cellular level from perspective of plant development and organogenesis

Content Delivery

Use of different experimental approaches will be demonstrated on the selected problems from developmental and cell biology. Focus will be on clear connection between experimental outcomes, interpretation of results and formulation of conclusions and hypothesis Interactive approach stimulating active participation of students on the solving presented biological questions

Coursework And Assignment Details

Students will be stimulated to active participation on the lectures;  common discussion and solving presented biological questions gives opportunity to every individual student to demonstrate their knowledge, independent thinking and ability to apply gain knowledge on concrete biological problem. Every day of lectures will be concluded by small colloquium focused on summarizing of topic of the day Written exam will be performed last day of lectures