DNA Methods for Authentication and Traceability of Agricultural and Food Products

Aims

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Prerequisites

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Learning Outcomes

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Syllabus

The rationale behind DNA-based authentication and traceability. The advantages and the challenges of DNA fingerprinting. Selection of DNA targets/markers for authentication and traceability. DNA barcoding, restriction fragment length polymorphisms (RFLP), sequence-tagged sites (STS), simple sequence repeats (SSR), sequence characterized amplified region (SCAR), amplified fragment length polymorphism (AFLP), single nucleotide polymorphisms (SNPs). Comparison and discriminative power of various DNA markers. Methods for DNA extraction. Issues concerning the quantity, quality, stability and complexity of isolated DNA. Polymerase chain reaction (PCR), real-time PCR, quantitative end-point PCR, multiplex PCR. Isothermal technologies. Digital PCR. High resolution melting analysis (HRM). Sequencing platforms. Automation and high-throughput systems for molecular testing. Multiplexing assays. Miniaturized systems. Manufacturing and performance of DNA chips. DNA Microarrays. Biosensors, (electrochemical, mass and optical). Nanoparticle-based devices. Devices suitable for field testing. Critical presentation of the various methods/devices. Standardization and validation of molecular methods.    

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Coursework And Assignment Details

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